Comrades true: TCU students take part in Horned Frog conservation
Saving the mascot, part two

TCU’s own Horned Frogs are making strides in saving the school’s mascot.
Since the Williams lab began studying horned lizards, students have contributed to conservation projects on the genetics, habitat and diet of Horned Frogs. More recently, they have begun to evaluate the reintroduction site to help the next generation of lizards survive.
Williams said the students in his lab have made some of the most significant discoveries for the horned lizard project.
“They have found out a lot of the cool stuff that we’re using now,” he said.
Some of the most surprising findings have come from Ph.D. student Rachel Alenius.
Alenius was on a study abroad trip to save rhinos in South Africa when she learned about efforts to protect the Texas horned lizard back at TCU.
She joined the Williams lab in 2015 as an undergraduate biology major, studying the lizards in the stifling summer heat of South Texas. The project would fulfill her Honors thesis, “but also I was just really excited to see a horned lizard,” she said.
Statewide, the horned lizard is considered a threatened species, but in the small towns of Kenedy and Karnes City, the lizards were abundant. Biologists wanted to know why.
In short, they adapted. After urban development and those pesky fire ants disrupted their food supply, researchers were surprised to find they changed their menu.
“Basically they were eating lots of really tiny ants and termites, which it’s not normal for adult Texas horned lizards to do that,” said Alenius.

A Karnes County horned lizard equipped with a radio transmitter to track her movements. Williams began exploring the diet of horned lizards in South Texas after Texas Parks and Wildlife brought up the population as a potential study subject. (Photo courtesy Dean Williams)
A Karnes County horned lizard equipped with a radio transmitter to track her movements. Williams began exploring the diet of horned lizards in South Texas after Texas Parks and Wildlife brought up the population as a potential study subject. (Photo courtesy Dean Williams)

A Texas horned lizard at the Fort Worth Zoo next to the harvester ants it eats. Harvester ants were once thought to comprise almost all of the lizards' diet. (Andrew Emery/TCU Marketing and Communication)
A Texas horned lizard at the Fort Worth Zoo next to the harvester ants it eats. Harvester ants were once thought to comprise almost all of the lizards' diet. (Andrew Emery/TCU Marketing and Communication)
Her research upended the belief that horned lizards depended on harvester ants like pandas depend on bamboo.
“[Alenius’s work] kind of opened up a whole new world in terms of understanding what exactly their dietary needs are,” said Williams.
Williams said fewer predators in town likely helped. Horned lizards usually depend on larger ants so they can eat quickly and hide from predators, but with fewer threats around, “They have maybe the luxury of sitting out in the open and eating small things,” he said.
Alenius' findings were so strange - and exciting - that she stayed on with Williams to pursue a master’s on the same topic.
Now pursuing her doctorate degree in biology, Alenius studies the diet and survival of hatchlings at Mason Mountain Wildlife Management Area in Central Texas, building conservationists’ knowledge of reintroduced horned lizards.
Horned lizard habitat
Twenty miles south of where Mason Mountain’s horned lizard population had disappeared, a rancher found lizards thriving on his property. Researchers set out to learn why.
“There’d been kind of this open question [as] to whether the habitat at Mason [Mountain] Wildlife Management Area was suitable,” said Williams.
Over the summer, graduate biology students Padraic Elliott and Patrick Ryan compared the habitats at the two sites to reveal whether Mason Mountain is a quality environment for horned lizards and how it could be improved.
Elliott studies plants, so when the Williams lab needed a student to research the vegetation at the two areas, “I jumped at that opportunity,” he said.
By examining the size, species and diversity of plants at Mason Mountain and the ranch site, his project adds “one more tool” to finding the secret sauce for successful reintroductions.
Ryan, who often collected data alongside Elliott, focused on the thermal habitat of the lizards.
Since lizards are ectotherms, or cold-blooded animals, they cannot regulate their body temperature to cool off and can die if temperatures rise above their “critical limit” of about 40 degrees Celsius, or 104 degrees Fahrenheit.
Ryan placed devices that record temperature in different “microhabitats” lizards use - dense vegetation, open areas and soil - at the ranch and Mason Mountain.
If the thermal habitats at the two sites are significantly different, “Kind of the assumption is that that natural population is the better habitat because it’s been sustaining this natural population, where at these reintroduction sites they can’t get a foothold there,” said Ryan.
Photo: A tagged Texas horned lizard rests near vegetation. (Photo courtesy Dean Williams)


A Texas horned lizard splays out next to a life-size thermal tracker that records temperatures at locations within Mason Mountain and the native ranch. Lizards use different habitats throughout the day to regulate their body temperature. (Photo courtesy Dean Williams)
A Texas horned lizard splays out next to a life-size thermal tracker that records temperatures at locations within Mason Mountain and the native ranch. Lizards use different habitats throughout the day to regulate their body temperature. (Photo courtesy Dean Williams)
Elliott, Ryan and Alenius also collected data on where horned lizards spent time at Mason Mountain to help conservationists choose the best release spots.
“That’s kind of like the overarching idea is just improving both reintroduction habitat and site selection so we can have better results to save the species,” said Ryan.
The students would follow the lizards every day, taking measurements of the habitat where each lizard was found and comparing it to a random point nearby to determine what environments the lizards prefer.
Ryan and Elliott focused on adult lizards, while Alenius tracked the hatchlings at the ranch and wildlife management area.
“We’re going to bring [all the projects] together to see if it’s habitat that’s hurting them,” said Ryan.
Following the Frogs

To study the lizards, the students have to find them first.
Adult lizards were caught and fitted with radio transmitters that broadcast a unique frequency for each lizard up to a kilometer away.
The frequencies are picked up as “beeps” by handheld receivers with antennas that help students determine the direction of the transmitter. The louder the sound, the closer the lizard.
“It’s like playing hot and cold,” said Ryan.
Hatchlings were too small for the radio transmitters, so researchers opted for another tracking device called harmonic radar. The team glued reflective tags attached to a strip resembling 35-millimeter film to the back of the lizards.
Rains said the harmonic tags were useful but had drawbacks for tracking the lizards.
“Like any reptile, they shed their outer skin off, so there’s a limited time we can track them,” he said.
The students attempted to find every tagged horned lizard once a day. Even with the tags, spotting them could be a challenge. If the tag had fallen off, the lizards were almost impossible to find.
Every day or two, the students would change the time they tracked an individual to make sure their data reflected the lizards’ daily patterns.
“We were working probably about nine, 10-hour days to make sure that our data was not biased or was taking into account the time of day,” said Elliott.
In addition to collecting data for their own projects, they also gathered information on the size, sex and age of the lizards and logged deaths from predators. It may sound simplistic, but it’s some of the first data of its kind.
“Because we’re dealing with reintroduction animals for our project, those haven’t been looked at before in this level of detail,” said Ryan.
Rains said the students’ role in tracking the lizards is one of the most important parts of the reintroduction project.
“That’s really the only way we can evaluate what’s happening to them,” said Rains.
Photo: A Texas horned lizard hatchling seems to "smile" for the camera. (Photo courtesy Padraic Elliott)


A coachwhip snake attempts to swallow a Texas horned lizard. When threatened, the lizards can swell to nearly twice their normal size to prevent predators from eating them. (Photo courtesy Patrick Ryan)
A coachwhip snake attempts to swallow a Texas horned lizard. When threatened, the lizards can swell to nearly twice their normal size to prevent predators from eating them. (Photo courtesy Patrick Ryan)
Not a dull day went by tracking the lizards.
One day, Ryan and Alenius caught up to an adult lizard they had been tracking - halfway down the throat of a coachwhip snake.
“Our principle is just let nature take its course,” said Ryan, “But what ended up happening in the moment was I was kind of like, ‘You know I need to get this tracker back.’”
Thinking the lizard was dead, Ryan and Alenius retrieved the lizard with plans of taking off the tracker. But the lizard was still breathing.
“It looked pretty jacked up,” said Ryan, but they kept the lizard indoors overnight and she recovered fully by the next day.
“I give her a day of not tracking her so it doesn’t bias the results as much, and then like a week later, she gets eaten again,” he said.
The research team waited until the snake passed the tracker through its digestive system and retrieved it. No harm, no foul - except for the lizard.
With so many lizards getting eaten, Alenius said some of her favorite moments working with horned lizards are when she finds an individual that had been missing and it is “way bigger than I would have expected.”
“To see how much it’s grown, it’s just such a pleasant surprise,” she said.
Students on the front lines at Mason Mountain also made the biggest discovery of the project so far: the first-ever clutches of wild hatchlings at the reintroduction site.
Last year, Alenius helped identify spots where females could be nesting. “Lo and behold, around the beginning of August, that’s when we started to find little hatchling horned lizards,” she said.
Alenius counted about 25 hatchlings and suspects there were even more she didn’t find.
The hatchlings are a unique milestone for reintroduction efforts in Texas. But Williams cautioned the project is far from over. “It’s a long hard row to hoe,” he said.
To save horned lizards, more work needs to be done - both in the field and in the classroom.
Photo: A baby horned lizard, smaller than a thumb. Finding horned lizard hatchlings in the wild gave conservationists hope for the reintroduction effort. (TCU Photo Library)

Saving the mascot
Across the nation, 31 universities have adopted the tiger as their mascot. But the TCU Horned Frogs are as unique as the lizard they’ve embraced as their own.
The reptile first became the official mascot in 1897, when the school yearbook was named “The Horned Frog.” Since then, the moniker has taken on a life of its own and been adopted by university athletics teams and fans alike.
But with the costumed mascots, t-shirts and foam fingers comes confusion for some fans.
TCU calls the lizard a “Frog,” a popular nickname because of the animal’s stout frame and lumpy body.
“I think it probably does add to the confusion, but [that’s not] necessarily a bad thing. I like that the mascot has so many funky names because it’s kind of a funky looking animal,” said Ryan.
Ryan said TCU also has the responsibility to educate students about the mascot. Students could learn about horned lizards in orientation and help with community outreach at nearby high schools, he suggested.
“I really have enjoyed raising awareness,” said Tucker, who taught in the College of Science and Engineering as part of her graduate student duties during her time with the Williams lab. “A lot of [students] don’t even know what a horned frog looks like; is it a frog, is it a lizard?”
Many Horned Frog fans do not realize how tiny the mascot is - at about four inches long, it’s 18 times smaller than the six-foot SuperFrog.
“TCU people think that it’s a fierce predatory horned lizard, right, because you see the football entrance and stuff. And then you pick them up and they’re kind of derpy,” Ryan said, laughing. “It's a great mascot, don’t get me wrong, but it’s not this fierce lizard, bringer of doom and destruction.”
Ryan is proud TCU takes part in protecting Texas horned lizards. “Not a lot of schools do conservation work on their mascot,” he said.
Tucker added that the Horned Frog’s threatened status adds a special connection for Frog fans.
“It’s kind of fun to work on a project that’s so closely linked to the university and kind of help with the conservation effort,” said Tucker.
Photos: TCU fans throw up the "Frogs" hand sign at the TCU football vs Baylor game in Amon G. Carter Stadium on Sept. 18, 2010 (Michael Clements/TCU Media Relations and Marketing); SuperFrog makes an appearance at orientation on July 20, 2021 (Andrew Emery/TCU Marketing and Communication); The Ol' Rip Horned Frog statue behind Scharbauer Hall (Camilla Price/Copy Desk Chief)



Light of faith
In spite of the challenges the project has faced, optimism for Texas horned lizards is on the rise.
The project is growing to include more stakeholders interested in saving the state reptile.
Historically, caring for the lizards was too expensive and time-consuming for most zoos. Fort Worth Zoo staff - who announced the hatching of their 1000th Texas horned lizard in September - are sharing care guidelines for the lizards with other institutions to help the breeding program expand.
The Dallas Zoo and Caldwell Zoo in Tyler began breeding horned lizards in the last three years, and Fossil Rim Wildlife Center in Glen Rose expressed interest in joining the project.
“The more partners we have, the more resources that we can share and use for each other to be successful,” said Barber.
This fall, the zoos reintroduced more than 260 hatchlings at Mason Mountain in the largest releases yet.
As the project has developed, students have also reaped the benefits.
Alenius said the opportunity to see how the Fort Worth Zoo and Parks and Wildlife staff operate “is just kind of neat for me professionally.”
For Ryan, the connections made through the Williams lab could last beyond his master’s: “There’s always a chance I’ll maybe do a gap year where I work for [Parks and Wildlife] for a year or so while I get things figured out,” he said.

A Texas horned lizard hatchling. (Photo courtesy Dean Williams)
A Texas horned lizard hatchling. (Photo courtesy Dean Williams)

Diane Barber (right) and the Fort Worth Zoo staff tag and prepare hatchlings for release on Sept. 16, 2021. (Photo courtesy Texas Parks and Wildlife)
Diane Barber (right) and the Fort Worth Zoo staff tag and prepare hatchlings for release on Sept. 16, 2021. (Photo courtesy Texas Parks and Wildlife)
Next up for the team: Refining the process.
The researchers will continue monitoring the lizards and teasing out the different variables affecting horned lizard survival at Mason Mountain.
Rains said they are hopeful for the reintroduced population. “The more we learn, the more we realize they’re a little more adaptable than we thought,” he said.

TPWD and Fort Worth Zoo staff process and tag hatchlings for release at Mason Mountain Wildlife Management Area in 2019. (Photo courtesy Texas Parks and Wildlife)
TPWD and Fort Worth Zoo staff process and tag hatchlings for release at Mason Mountain Wildlife Management Area in 2019. (Photo courtesy Texas Parks and Wildlife)
If the population stabilizes - which could be in five years or thirty - conservationists will expand the reintroduction effort to other sites where horned lizards once roamed.
Rains doesn’t have a particular place in mind, but he’s not worried about finding locations: “I can imagine for years we’d just get flooded with calls about people wanting to be on the mythical 'list,' ” he said.
Fort Worth residents with visions of football fields covered in horny toads may be in for disappointment.
“We’ll never be able to restore horned lizards to where they used to occur all over the state,” said Rains.
Reintroduction efforts cost time and money, and in many areas, the habitat is too far gone for lizards to establish a population.
But Rains still plans to keep the connection between Texans and horned frogs alive.
By reintroducing lizards to public sites, “People could take their family to see a lizard that’s within driving distance,” he said.
Although the conservation efforts continue, Alenius’s journey with the Williams lab could be coming to an end.
“I feel really blessed to be able to have found myself in this position where I’ve gotten to work with so many really, really fascinating people,” she said.
For her, the project also provides something more intangible.
“When I started this, I was coming off that trip from South Africa where I honestly felt very discouraged… I felt like there wasn’t a lot I could personally do to help,” she said. “And to see that what I’m doing with horned lizards is having a direct effect on their conservation, on their management strategies, has been just very affirming… Going out there every day, even though it’s hard, is paying off, and that’s really, really cool.”
To keep up with the adventures of the Williams lab, follow the TCU Horny Toad Project on Facebook.

The Ol' Rip statue behind Scharbauer Hall was installed in 2012 and named for the legendary horned lizard that survived being buried in a time capsule under a Texas courthouse for 31 years. Horned lizards are an integral part of the TCU campus. (Camilla Price/Copy Desk Chief)
The Ol' Rip statue behind Scharbauer Hall was installed in 2012 and named for the legendary horned lizard that survived being buried in a time capsule under a Texas courthouse for 31 years. Horned lizards are an integral part of the TCU campus. (Camilla Price/Copy Desk Chief)